ABSTRACT
Leptadeniahastata (Pers.) Decne (Asclepiadaceae) is widely African plant species used in traditional medicine as an anti-venom, antihypertensive, anti-diabetic, analgesic and also used for catarrh and skin disease. The powdered leaves of L. hastatawas extracted with methanol using maceration method and the resulting crude methanol extract (ME) was suspended in water and fractionated into hexane (HF), chloroform (CF), ethylacetate (EF) and n-Butanol (BF). Preliminary phytochemical screening of ME revealed the presence of carbohydrates, alkaloids, flavonoids, anthraquinones, saponins, tannins, glycosides, steroids and triterpenes while (HF) revealed the presence of steroids and triterpenes nucleus. HF was step-wisely eluted in a silica gel packed column to afford ten fractions H1-H10 and fraction H4 was selected for further purification using Preparative Thin Layer Chromatography, with the solvent system of hexane: ethyl acetate (8.5 : 2.5). This resulted in the isolation of a whitish crystalline compound M1 (βSitosterol). The structure of the compound was elucidated using chemical test and spectroscopic techniques (IR, 1D & 2D-NMR) and by comparison with reference spectral data. The LD50 of the methanol extract (ME) and the LD99 of the Echisocellatus venom were estimated to be greater than 5000 mg/kg and 2.2 mg/kg respectively. Anti-venom studies suggest that ME possess significant anti-venom activity against Echisocellatusvenom both In vitro and In vivo. Analgesic effect was evaluated using acetic acid induced writhing in mice. The results showed that ME (150 mg/kg) decreased writhing response with 84.6 % inhibition. However, the extract when compared to control showed statistically significant difference at (p˂0.05)
ABSTRACT
The study centers on the problems militating against effective teaching and learning of Accounting education in...
EXCERPT FROM THE STUDY
According to Dike (2005), Vocational Education and training “prepares learners for careers...
BACKGROUND OF THE STUDY
Though many people would agree that the standard of education in Nigeria has de...
ABSTRACT
Evaluation is a prelude to strategic action. Strategies once identified must definitely be evaluated, so as to...
Abstract
The objective of the present study was to evaluate the antidiarrheal activity of the methanol stem bark extract of Hymenocardia...
BACKGROUND OF THE STUDY
The use of information and communications technology (ICT) to improve and/or as...
BACKGROUND TO THE STUDY
Presently, the crisis of development is the most seri...
Abstract
The aim of this study was to investigate Audience perception of federal government removal of...
INTRODUCTION
Punctuation is one of the major aspects that contribute to meaning of any written discourse. This implies t...
Background of study
Richard A. Joseph… is usually credited with first using the term prebendalism to describe...